基于石墨烯纳米复合物的无标记免疫传感器，用于同时多重电化学测定肿瘤标志物
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2013-09-30 , DOI: 10.1016/j.bios.2013.09.050
Xinle Jia , Zhimin Liu , Na Liu , Zhanfang Ma
在这里，我们制备了一种无标记的电化学免疫传感器，采用氧化铟锡（ITO）薄板作为工作电极，石墨烯纳米复合材料作为支持基质，用于同时测定癌胚抗原（CEA）和甲胎蛋白（AFP）。合成了还原的氧化石墨烯/硫氨酸/金纳米颗粒，并将其涂覆在ITO上以固定抗CEA，同时使用还原的氧化石墨烯/普鲁士蓝/金纳米颗粒固定了抗AFP。免疫传感器的确定基于以下事实：由于抗体-抗原免疫复合物的形成，硫氨酸和普鲁士蓝的响应电流降低与相应抗原的浓度成正比。-1。CEA的检出限为0.650 pg mL -1，而AFP的检出限为0.885 pg mL -1。此外，该方法已被评估用于临床血清样品的分析，并与酶联免疫吸附测定法具有良好的相关性。
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A label-free immunosensor based on graphene nanocomposites for simultaneous multiplexed electrochemical determination of tumor markers
Here we prepared a label-free electrochemical immunosensor employing indium tin oxide (ITO) sheets as working electrodes and graphene nanocomposites as supporting matrix for simultaneous determination of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP). Reduced graphene oxide/thionine/gold nanoparticles nanocomposites were synthesized and coated on ITO for the immobilization of anti-CEA while reduced graphene oxide/Prussian Blue/gold nanoparticles were used to immobilize anti-AFP. The immunosensor determination was based on the fact that due to the formation of antibody–antigen immunocomplex, the decreased response currents of thionine and Prussian Blue were directly proportional to the concentrations of corresponding antigens. Experimental results revealed that the multiplexed immunoassay enabled the simultaneous determination of CEA and AFP with linear working ranges of 0.01–300 ng mL−1. The limit of detections for CEA is 0.650 pg mL−1 and for AFP is 0.885 pg mL−1. In addition, the methodology was evaluated for the analysis of clinical serum samples and received a good correlation with the enzyme linked immunosorbent assay.
更新日期：2013-09-30