Electrochimica Acta ( IF 5.5 ) Pub Date : 2020-07-27 , DOI: 10.1016/j.electacta.2020.136828
Qingqing Zhang , Tingting Hao , Dandan Hu , Zhiyong Guo , Sui Wang , Yufang Hu
基于辅酶A（CoA）引发的竞争反应和先进的DNA纳米生物技术，精心设计了一种新的涉及适体的组蛋白乙酰转移酶（HAT p300）RNA适配体电化学发光（ECL）策略。首次，这一有趣的分析过程包括由HAT p300催化的重要组蛋白乙酰化，CoA及其RNA适体的特异性结合触发的竞争性替代，DNA纳米棱镜引导的稳定支架以及杂交链反应管理的典型扩增（HCR）。一旦识别出乙酰化中产生的针对CoA的RNA适体，就会从适体/ A-DNA复合物中释放出辅助DNA（A-DNA），该辅助DNA可以被固定在Au电极表面的人造DNA纳米棱镜的三个悬臂捕获。此外，A-DNA还可以巧妙地打开一个发夹DNA（H1），然后通过与其他发夹DNA（H2）交替杂交进入HCR过程。丰富的Ru（phen）与dsDNA具有强相互作用的3 2+分子可产生大量输出。该平台对HAT p300具有极好的特异性和灵敏度，检出限为0.001 nM，具有出色的实用性和优越性，为生物医学中HAT p300分析提供了巨大的潜力。
"点击查看英文标题和摘要"
RNA aptamer-driven ecl biosensing for tracing histone acetylation based on nano-prism substrate and cascade dna amplification strategy
A new RNA aptamer-involved electrochemiluminescence (ECL) strategy for sensitive detection of histone acetyltransferase (HAT p300) is designed well based on coenzyme A (CoA)-initiated competitive response and advanced DNA nano-biotechnology. For the first time, this interesting analytical process is comprised of significant histone acetylation catalyzed by HAT p300, competitive replacement triggered by the specific binding of CoA and its RNA aptamer, stable scaffold guided by DNA nano-prism and typical amplification managed by hybrid chain reaction (HCR). Upon recognition of the RNA aptamer to CoA generated in acetylation, auxiliary DNA (A-DNA) which can be captured by three hanging arms of the fabricated DNA nano-prism immobilized onto Au electrode surface is released from the aptamer/A-DNA complex. Moreover, A-DNA can also open skillfully one hairpin DNA (H1), then turning into HCR process by alternating hybridization with the other hairpin DNA (H2). Abundant Ru(phen)32+ molecules which have a strong interaction with dsDNA can result in a significant output. This proposed platform exhibits excellent specificity and sensitivity toward HAT p300 with a detection limit of 0.001 nM and takes on outstanding practicality and superiority, which presents a great potential for HAT p300 analysis in biomedical sciences.
更新日期：2020-08-03